Avaliação do teor de ésteres de phorbol nas sementes das variedades híbridas de Jatropha curcas L.

Abstract

The expansion of the Jatropha curcas L. production chain demands the development of genotypes with characteristics that meet the needs of the productive, social, environmental and technological development in the country. The presence of phorbol esters in different parts of the plant raises professional and ecotoxicological concerns due to its toxicity. Considering the above, in this work it was evaluated the content of phorbol esters in the albumen and in the inner bark of the varieties hybrids of Jatropha. The extraction of the phorbol esters was done by maceration with methanol under stirring in Vortex at 1800 rpm and magnetic at 600 rpm for 15 minutes, for the inner shell and albumen, respectively. The extracts were analyzed by High Performance Liquid Chromatography (CLAE) with the Visible Ultraviolet (UV-Vis) detector at 282 nm. The content of phorbol esters in the inner skin ranges from 2.14-2.91mg / g in female varieties, 1.73- 2.82 mg / g in hybrid varieties and 0.60-1.54 mg / g in male varieties. In albumen, the levels of phorbol esters vary between 0.46-1.22 mg / g in female varieties, 0.29-1.04 mg / g in female varieties hybrid varieties and 0.28-0.96 mg / g male varieties. The results showed that the levels of phorbol esters in hybrid varieties in the bark internal and albumen tend to the artimetic average of the content of their male and female parents, the unimodal ANOVA test illustrates a value of p <0.05 in the inner shell and albumen. Therefore, you accept the alternative hypothesis that states that there are significant differences between the means in the varieties study.