Efeito do balanço de concentrações dos reguladores de crescimento BAP e ANA na indução de callus a partir de embriões zigóticos de Millettia stuhlmannii Taub. (Jambire)

Abstract

The research investigated how different concentrations of the growth regulators BAP (6- benzylaminopurine) and ANA (naphthaleneacetic acid) affect callus formation and regeneration of somatic embryos in explants of Millettia stuhlmannii Taub. embryos, an important timber company known as jambire. This study aims to contribute to the reforestation, considering the massive cutting of the species and climate change. THE general objective was to evaluate the impact of different concentrations of BAP and ANA on the formation of callus and in the induction of somatic embryos in Millettia stuhlmannii Taub. Specifically, we sought to identify the time required for callus formation and its morphology, compare the effectiveness of different concentrations of growth regulators in the formation of callus. Embryos of Millettia stuhlmannii Taub. were excised from seeds and inoculated in culture media containing various combinations and concentrations of BAP and ANA, varying from 0.5 to 2.0 mg/L for BAP and from 1.0 to 3.0 mg/L for ANA, in a dark environment at 24ºC. THE callus development and somatic embryo formation were monitored for 8 weeks. Specific combinations of BAP and ANA were effective in inducing callus and somatic embryos. embryogenic callus began to form in the third week, presenting a yellow, transparent color and a compact and friable texture. The presence of contamination affected the formation of callus and embryos and explants. The BAP regulators and ANA have demonstrated efficacy in the formation of callus and somatic embryos, although the balance between them did not show any significant difference, demonstrating that embryos of this species are good explants and that the regulators are suitable for callus and embryo formation somatic. These results contribute to the establishment of in vitro culture protocols. for callus and facilitate the propagation and conservation of the species.