Detecção molecular de Ehrlichia Ruminatium nos diferentes estágios de crescimento da Amblyomma Hebraeum

Abstract

Ehrlichia ruminantium, the bacterium that causes ehrlichiosis, is transmitted by ticks of the genus Amblyomma. The molecular detection method called reverse line blotting (RLB), which consists of hybridization using oligonucleotide probes with the region of conserved domains within the 16S rRNA gene called hypervariable loop VI amplified using the polymerase chain reaction (PCR) , was used to compare the frequencies of A. hebraeum infection in three different growth stages (larvae, nymphs and adults). A total of 110 samples were collected, of which 39 larvae and 39 nymphs of A. hebraeum were obtained from the campus of the Faculty of Veterinary Medicine of Universidade Eduardo Mondlane, and 32 adult ticks of the same species were collected from the fur of cattle at different points in the district of Matituíne. Hybridization of the positive controls with the respective probes showed that the RLB process was carried out successfully, however, all samples tested were negative, which primarily suggests the absence of the microorganism in the samples studied (TRADUÇÃO NOSSA)