Caracterização das estirpes de neisseria gonorrhoeae em Moçambique: auxotipagem, serotipagem e concentração inieitoria mínima

Abstract

A total of 151 non-selective Neisseria gonorrhoeae strains, isolated from urethral discharge samples collected from male patients consecutively observed in the consultations of dts in the city of Maputo, were sent from the national reference laboratory of d.t.s. in Mozambique for the Institute of Tropical Medicine in Antwerp-Belgium. Of these, 137 strains of Neisseria gonorrhoeae were recultured and tested for their sensitivity to seven (7) antibiotics, by the agar dilution method, to determine the minimum inhibitory concertation; auxotyping was done according to the catlin methodology and serotyping according to the sandstrom and danielson methodology using the knapp nomenclature. Ninety (65.7%) of the isolated strains produced b-lactamase (ppng) and 47 (34.8%) did not produce b-lactamase (non-ppng). 68.9%) were considered strictly ppng and 28 (31.1%) ppng highly resistant to tetracycline (ppng/trng). From Mao-ppng, 1 strain (2.13%) showed chromosomal resistance to penicillin; 21 (44 .7%) showed chromosomal resistance to tetracycline; and 5 (10.6%) showed chromosomal resistance to penicillin and tetracycline. One (1) strain was highly resistant to tetracycline (trng) and showed chromosomal resistance to thiamphenicol. 76 (55.4%) were resistant to thiamphenicol. All strains were sensitive to kanamycin, spectinomycin, ciprofloxacin and ceftriaxone. Twenty different serovars were detected, the most frequent being: ia6 with 39 (28.5%) strains and ia2 with 15 (11%)strains from serogroup wi and ib3 with 22 strains (16.1%), ib5 with 15 (11%) strains and ib23 with 8(5.8%) strains from serogroup wii/wii. Nine auxotypes were found, the most prevalent being: pro- with 57 (41.6%) strains, proto with 50 (36.4%) strains, pro-,hypo-,ura-(phu-) with 12(8.8%) %) strains and hypo-,ura- (hu-) with 7(5.1%) strains