Estudos dos óleos essenciais da cupressus por cromatografia gasosa acoplada a espectrometria de massas

Abstract

The present work was concerned with the study of new plants that produce essential oils, once the study of eucalyptus, thuja and juniperus is finished. Cupressus, a plant originating in Europe, can with great merit be grouped with other plants already studied, although investigations into it have not been ended because this plant, in addition to the monoterpenes referred to in the following pages, also contains such sesquiterpenes. such as caryophyllene, cadalene, bisabolene, and eudalene. Cupressus is a sample that contains almost all monoterpenoids and can therefore be considered the basis for the other samples. The work gained its importance not only for the proper choice of the sample but also for the excellent methodical approach with which it was conducted by the supervisor in the search for different ways to identify the eluted components of the GC. Some of these ways include mass spectrometry, comparison to GC standards, applicability of Kovats indices in compound identification, and sometimes available retention data curves. The study was carried out in four stages: 1- Study of the oil composition in two columns (DBWax and DB-5); 2- Study of the composition of non-condensed vapors at a temperature of 10 °C; 3- Study of hydrolates; 4- Study of the composition of vapors when using the SPME technique. With this study it was concluded that only in the oil and in the vapors obtained by the SPME technique the monoterpenes appeared, while the hydrolates contained alcohols and the vapors contained ketones and epoxides. In order to test the extraction efficiency, the Kissue extractor was used in addition to the Clevenger hydrodistiller and the results point to an efficient yield when using the Kissue extractor. This means that after applying the "t" student test to compare the means given by the two devices and the "F" test to compare their standard deviations, the results were in agreement: at a confidence level of 95%, there is significant evidence that the yields given by the two devices differ significantly (TRADUÇÃO NOSSA)