Determinação quantitativa simultânea de Sulfadoxina e Pirimetamina no Fansidar

Abstract

Malaria remains the most devastating human infection worldwide with 300 to 500 million clinical cases and nearly 3 million deaths per year. In Mozambique about 48% of all external consultations and about 63% of all pediatric admissions to rural and central hospitals were attributed to malaria. Nowadays, in malaria chemotherapy, Fansidar is used, which is a combination of Sulfadoxine (500mg) and Pyrimethamine (25mg) that inhibits the subsequent steps involved in the biosynthesis of tetrahydrofolic acid. The low quality of medicines is the biggest public health problem. Especially in developing countries where there is a lack of adequate resources to effectively control these problems. Outdated or counterfeit use of Sulfadoxine and Pyrimethamine delays chemotherapeutic treatment for malaria. It is with these considerations that this work describes a simple, fast, cheap and sensitive colorimetric authentication method for these antimalarials. Sulfadoxine and Pyrimethamine were isolated from the injectable Fansidar using their solubilities, Sulfadoxine being water soluble and Pyrimethamine insoluble. Then followed the purification and analysis of the melting points of each compound, obtaining about 198-201 °C for Sulfadoxine and 240-243 °C for Pyrimethamine, values ​​very close to literature data fxzs.js .-m Purity analysis was done using Thin Layer Chromatography (TLC) The confirmation of the structures was done using Infrared spectra and mass spectra, having obtained bands that correspond to the desired compounds (Sulphadoxine and Pyrimethamine). Sulfadoxine and Pyrimethamine absorb in almost the same region (200-400 nm for Pyrimethamine and 230-350 nm for Sulfadoxine), making simultaneous determination in pharmaceutical formulations impossible without first separating [13, IJ]. (TRADUÇÃO NOSSA)